Browsing by Subject "culture medium"
Now showing items 1-12 of 12
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The activity of three glycosidases (β-Ν-acetyloglucosaminidase, α-mannosidase, and β-galactosidase) in the follicular fluid and in the maturation medium affects bovine oocyte maturation
(2016)We studied the role of follicular fluid's (FF) glycosidase (α-mannosidase [α-ΜΑΝ], β-Ν-acetyloglucosaminidase [NAGASE], β-galactosidase [β-GAL]) activity during IVM of bovine oocytes. Oocytes were allocated into two groups ... -
Application of a semi-dependent latent model in the Bayesian estimation of the sensitivity and specificity of two faecal culture methods for diagnosis of paratuberculosis in sub-clinically infected Greek dairy sheep and goats
(2006)In this study, we compared the frequency of isolation of Mycobacterium avium subsp. paratuberculosis (MAP) from faecal samples grown on Herrold's egg-yolk medium (HEYM) or on Lowenstein-Jensen (LJ) medium and estimated the ... -
Comparison of isotonic activation of cell volume regulation in rat peritoneal mesothelial cells and in kidney outer medullary collecting duct principal cells
(2021)In disease states, mesothelial cells are exposed to variable osmotic conditions, with high osmotic stress exerted by peritoneal dialysis (PD) fluids. They contain unphysiologically high concentrations of glucose and result ... -
A comparison of statistical approaches used for the optimization of soluble protein expression in Escherichia coli
(2016)During a discovery project of potential inhibitors for three proteins, TNF-α, RANKL and HO-1, implicated in the pathogenesis of rheumatoid arthritis, significant amounts of purified proteins were required. The application ... -
Considerations regarding embryo culture conditions: From media to epigenetics
(2018)There are numerous reports on embryo culture media and conditions in the laboratory, as the subject is multifaceted and complex, reflecting the variation in practice. In this scoping review, we attempt to approach the topic ... -
Effect of macronutrients and of anaerobic digestate on the heterotrophic cultivation of Chlorella vulgaris grown with glycerol
(2022)The aim of this work was to investigate the kinetics of the heterotrophic growth of Chlorella vulgaris as a means of producing bio-oil for biodiesel production. Glycerol was used as the sole organic carbon substrate. ... -
Efforts to create an artificial testis: Culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment
(2005)Induction of meiotic and post-meiotic alterations of male germ cells in vitro has been the target of several research efforts since 1960. However, to date, the establishment of an ideal culture system in which spermatogonial ... -
Genes and conditions controlling mammalian pre-and post-implantation embryo development
(2015)Embryo quality during the in vitro developmental period is of great clinical importance. Experimental genetic studies during this period have demonstrated the association between specific gene expression profiles and the ... -
Preconditioning of primary human renal proximal tubular epithelial cells without tryptophan increases survival under hypoxia by inducing autophagy
(2017)Purpose: Hypoxia plays a significant role in the pathogenesis of acute kidney injury (AKI). Autophagy protects from AKI. Amino acid deprivation induces autophagy. The effect of l-tryptophan depletion on survival and autophagy ... -
Recovery of Salmonella enterica from seropositive finishing pig herds
(2003)The aim of this study was to assess the probability of detecting Salmonella from pen faecal samples in seropositive classified finishing pig herds. The study involved 77 herds from Denmark (20), The Netherlands (20), Greece ... -
Responses of sweet pepper (Capsicum annum L.) cultivated in a closed hydroponic system to variable calcium concentrations in the nutrient solution
(2021)BACKGROUND: The use of water containing calcium bicarbonate (Ca(HCO3)2) at excessively high concentrations in closed hydroponic crops can cause calcium ion (Ca2+) accumulation in the recycled nutrient solution (NS) and ... -
Wet chemical silver treatment of endotracheal tubes to produce antibacterial surfaces
(2007)Mechanically ventilated patients in hospitals are subjected to an increased risk of acquiring nosocomial pneumonia that sometimes has a lethal outcome. One way to minimize the risk could be to make the surfaces on endotracheal ...